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81.
Alleviation of dormancy in walnut kernels by moist chilling is independent from storage protein mobilization 总被引:1,自引:0,他引:1
We studied the effects of moist chilling and warming on storage protein mobilization in walnut (Juglans regia L.) kernels to assess the metabolic inhibition theory, which states that dormant seeds are unable to utilize their own food reserves and that cold conditions allow germination by activating hydrolases involved in reserve mobilization. Stratifying kernels at 5 degrees C for 40 days enhanced their germination. During both cold stratification and warm incubation of kernels, storage protein mobilization occurred in cotyledons rather than axes. Kernel amino acid concentration increased during protein mobilization, with axes of warm-incubated kernels having particularly high amino acid concentrations. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE) of the soluble protein fractions from both cold-stratified and warm-incubated cotyledons revealed increased band intensities of putative glutelins (19-22 and 32-35 kDa). A very high molecular weight protease was detected by gelatin SDS-PAGE that was most active at acid to neutral pHs in imbibed, cold stratified and germinated kernels suggesting the protease(s) was synthesized earlier in the mature seeds. Thus, in dormant walnut kernels there is no block to protein mobilization, and imbibition alone is sufficient to initiate proteolysis. Catalase activity was higher in warm-incubated kernels than in cold-stratified kernels, suggesting that seed aging is hastened under warm conditions and that cold stratification in walnut kernels might involve activation of cellular repair mechanisms. 相似文献
82.
Alcohol and aqueous extracts of flowers of Azadirachta indica were tested in vitro for their potential antifilarial activity against whole worm, nerve muscle (n.m.) preparation and microfilariae of Setaria cervi. The effects of alcohol and aqueous extracts were similar in nature on the spontaneous movements of whole worm and nerve muscle preparation. On the whole worm, the response was characterized by initial increase in tone, rate and amplitude of contractions followed by reversible paralysis. The initial stimulant effect is likely to be due to irritant effect on the cuticle. Nerve muscle preparation responded to both extracts by inhibition of spontaneous movements followed by reversible paralysis; initial stimulation phase was absent. The inhibition was concentration related. Alcohol and aqueous extracts had almost similar lethal effect on the microfilariae of S. cervi, the LC50 being 15 and 18 ng/ml, respectively. 相似文献
83.
The predatory mite Typhlodromus pyri Scheuten (Acari, Phytoseiidae) has been reported as an important predator of the European red mite, Panonychus ulmi (Koch) (Acari, Tetranychidae) in apple culture and vineyards at below 25?°C. However, sufficient biological data was lacking on its efficiency at temperatures above 25?°C. Therefore, the purpose of the present laboratory work was to obtain experimental data on prey consumption during development as well as longevity and reproduction of T. pyri on apple leaf discs and in Plexiglas cells at constant temperatures of 25?±?2?°C and 30?±?2?°C with P. ulmi as prey. 相似文献
84.
Antibacterial activity of Barringtonia racemosa 总被引:3,自引:0,他引:3
The antibacterial activity of the ethanol extract of Barringtonia racemosa roots, its chloroform soluble fraction and, a there from an isolated clerodane diterpenoid is reported. 相似文献
85.
Antibacterial activity of Picrasma javanica 总被引:1,自引:0,他引:1
The methanol extracts of Picrasma javanica, leaves, seeds, stem and root barks were partitioned (petrol, dichloromethane, ethyl acetate, butanol). All obtained extracts and fractions showed a broad spectrum of antibacterial activity, while none was active against the tested moulds. 相似文献
86.
To optimize biomass and crude protein (CP) production for leucaena (Leucaena leucocephala) in southern Texas, field trials
were conducted with treatment combinations of three levels of phosphorus (P) , two levels of Mg and two levels of soluble
trace element mixture (STEM). The P was banded in the soil while the Mg and micronutrient blend were applied to the foliage.
A combination of P (22 kg ha-1), Mg and STEM fertilizers significantly increased biomass from 2555 kg ha-1 to 3028 kg ha-1. This treatment was associated with an increase in leaf CP from 27.9% to 31.0%. P fertilizer had no significant effect on
leaf P, but significantly increased leaf nitrogen (N) and leaf copper (Cu). A foliar spray of Mg significantly increased leaf
N, P, and Cu. A foliar spray of a complete micronutrient blend increased leaf Cu. Biomass production was correlated positively
(decreasing order) with leaf Cu, N, and Mg and negatively with Zn. Leaf N was positively correlated with leaf P and calcium.
The critical tissue concentrations (CTC) (where 90% of predicted maximum leaf protein was obtained) were 0.18% and 4.49 mg
kg-1 for leaf P and Cu, respectively.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
87.
The mean values of the total number of nematodes found in each of four pine plantations in Nigeria were depressed during the dry season to a minimum between March and April. A gradual increase in the population started from June–July, and the population reached its peak in August–October. The seasonal decline in nematode population coincided with the period of low soil moisture. An important ecological factor affecting population changes in the selected plantations was the vegetation cover; while a gradual decline in nematode population was observed in the 1968, 1974a and 1974b plantations, which have a full canopy, a sharp decline in nematode population was observed in the 1982 plot, which did not have a complete canopy. Populations of some nematode genera, such as Rotylenchus spp., Criconemella spp., Coslenchus spp. and Triversus spp., declined to zero in this last plantation. 相似文献
88.
Prof. Dr. M. A. Ali A. S. El-Khouly G. M. Moawad M. G. M. Ragab 《Journal of pest science》1992,65(3):51-54
Infection rates of the nuclear polyhedrosis virus (NPV) in the cotton leafworm,Spodoptera littoralis larvae were assessed from 1985 through 1986 in clover fields at El-Behiera, El-Fayoum and El-Gharbia governorates. Infection rates of the pathogen were highest near the end of spring when the cotton leafworm larval populations were increasing. For the different governorates, the percentages of virus infection were almost higher in 1985-season than in 1986. El-Fayoum-larval population was the most infected by NPV followed by El-Gharbia and El-Behiera-populations, respectively. There was a positive correlation between infection rates of NPV on one hand, and density of larva and ambient temperature on the other. 相似文献
89.
Pseudomonas solanacearum (Smith) was consistently isolated from the wilted plants of Casuarina equisetifolia. The isolate gave positive reaction to oxidase, catalase and gas production from nitrate and negative reaction for arginine dihydrolase and acid was produced from various carbohydrates. Wilt produced on tomato, egg plant and capsicum indicated that the pathogen belongs to Race I and Biovar III of P. solanacearum which is a new record from India. 相似文献
90.